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<div id="prlnh5h7t" class="kjkjb"> <div id="prlnh5h7t" class="bm vw" style="margin-bottom:0;"> <div id="prlnh5h7t" class="h hm"> <div id="prlnh5h7t" class="zxtoux"><img src="http://www.funny6.com/uc_server/avatar.php?uid=54876&size=small" /></div> <h1 class="ph">新型功能化熒光傳感器可實時高靈敏度檢測腸道病毒RNA </h1> <p class="xg1"> 時間：2025-4-11 09:05<a class="zz" href="home.php?mod=space&uid=54876">作者：update</a> 閱讀<em id="_viewnum">(60)</em> 評論: <a href="http://www.funny6.com/portal.php?mod=comment&id=10239&idtype=aid" title="查看全部評論"><em id="_commentnum">5</em></a></p> <div id="prlnh5h7t" class="zxfb" ><a href="http://www.funny6.com/portal.php?mod=rss&catid=57" class="y xi2 rss" target="_blank" title="RSS">訂閱</a></div> </div> <!--[diy=diysummarytop]--><div class="prlnh5h7t" id="diysummarytop" class="area"></div><!--[/diy]--> <div id="prlnh5h7t" class="s"> <div id="prlnh5h7t" class="ss"><div><span>導讀： </span>一種新型功能化熒光傳感器可實時高靈敏度檢測腸道病毒RNA 。這標志著更安全、更有效的病毒診斷邁出了重要一步。芬蘭于韋斯屈萊大學納米科學中心 (NSC) 的研究人員取得了一項重大突破，開發出一種創新的無標記比例熒 ...</div> </div> <!--[diy=diysummarybottom]--><div class="prlnh5h7t" id="diysummarybottom" class="area"></div><!--[/diy]--> <div id="prlnh5h7t" class="d"> <!--[diy=diycontenttop]--><div class="prlnh5h7t" id="diycontenttop" class="area"></div><!--[/diy]--> <table cellpadding="0" cellspacing="0" class="vwtb"><tr><td id="article_content"><div> 一種新型功能化熒光<a target="_blank" class="relatedlink">傳感</a>器可實時高靈敏度檢測腸道病毒RNA。這標志著更安全、更有效的病毒診斷邁出了重要一步。芬蘭于韋斯屈萊大學<a target="_blank" class="relatedlink">納米</a>科學中心(NSC)的研究人員取得了一項重大突破，開發出一種創新的無標記比例熒光傳感器，能夠高靈敏度和高選擇性地檢測腸道病毒RNA。</div><div><br></div><div><p style="text-align: center;"><a href="data/attachment/portal/202504/11/090503myyd68x0d6960yay.jpg" target="_blank"><img src="data/attachment/portal/202504/11/090503myyd68x0d6960yay.jpg"></a></p></div><div style="text-align: center;"><br></div><div style="text-align: center;"> 基于功能化碳點(CD)和溴化乙錠(EB)的比例型熒光傳感器（Func傳感器）的工作原理圖。圖片來源：JussiToppari教授繪制</div><div><br></div><div> 這一進展代表了病毒診斷領域的重大進步，并凸顯了跨學科合作在應對全球健康挑戰中的價值。</div><div><br></div><div> 病毒持續對公眾健康構成嚴重威脅，正如近期的疫情大流行所見。早期發現和準確識別對于控制感染的傳播至關重要。傳統的診斷方法雖然有效，但它們往往無法提供有關病毒基因組釋放的詳細時空信息，從而限制了其在動態或早期檢測場景中的應用。</div><div><br></div><div> “這項跨學科的研究融合了生物學、化學和<a target="_blank" class="relatedlink">物理</a>學的專業知識，標志著病毒檢測技術的重大進步。我們開發了一種增強型比率熒光傳感器，該傳感器使用探針（單鏈互補寡核苷酸片段）和溴化乙錠（EB）功能化的碳點（CD），用于檢測腸道病毒RNA。”于韋斯屈萊大學物理學教授JussiToppari說道。</div><div><br></div><div> 熒光納米粒子已成為生物分析物傳感的有力工具，而碳點憑借其簡單的合成、優異的光穩定性、可調的光致<a target="_blank" class="relatedlink">發光</a>、優異的水溶性、生物相容性以及用于配體結合的多種表面功能，在生物傳感領域處于領先地位。這些獨特的特性使碳點有望成為生物傳感領域的顛覆者。</div><div><br></div><div> “這種所謂的功能化傳感器（FuncSensor），其中CD被功能化，即與探針共價結合，明顯優于更傳統的非功能化傳感器（Non-FuncSensor），后者是CD、探針和EB的簡單混合物，”于韋斯屈萊大學博士研究員AmarRaj解釋道。</div><div><br></div><div> 在這兩種傳感器中，目標DNA與探針雜交，增強了EB熒光，而CD熒光由于<a target="_blank" class="relatedlink">電子</a>轉移而略有變化，從而實現比率檢測，并且具有超靈敏性。</div><div><br></div><div> 博士后研究員AbhishekPathak表示：“非功能性傳感器對目標DNA的靈敏度較低，對真正的腸道病毒RNA樣本無效；而功能性傳感器對DNA和真正的病毒RNA的靈敏度更高，選擇性明顯提高。”他之前曾在于韋斯屈萊大學擔任博士后研究員。</div><div><br></div><div> Func傳感器的卓越性能歸功于共價功能化帶來的增強電荷轉移。</div><div><br></div><div> “我們的原理驗證研究強調了探針共價固定對于改善CD和EB之間電子轉移的重要性，從而提高了性能，并證明了Func傳感器在快速、實時和精確原位檢測病毒RNA的實際應用中的適用性，”于韋斯屈萊大學細胞和分子生物學教授VarpuMarjomäki說道。</div><div><br></div><div> 尤其是研究表明Func傳感器可以在體外實時檢測衣殼中腸道病毒RNA的釋放。Marjomäki說：“這意味著Func傳感器可以用作一種新型病毒RNA傳感平臺，為檢測感染期間實時病毒RNA的出現提供了急需的可能性。”</div><div><br></div><div> 這項突破性的研究引入了一種檢測病毒RNA的新方法，并為熒光團之間的電荷轉移機制提供了新的見解。基于這些發現，該團隊目前正致力于用更安全、細胞毒性更低、生物相容性更高的替代品取代具有潛在危險性的染料溴化乙錠，從而提高該系統的安全性和可靠性。</div><div><br></div><div> Pathak高興地表示：“這一改進將進一步提高體內病毒RNA檢測的安全性和有效性。”</div><div><br></div><div><p microsoft="" yahei",="" simsun;="" background-color:="" rgb(255,="" 255,="" 255);="" text-align:="" center;"="" 255);"="" center;="" style="word-wrap: normal; margin: 0px; padding: 0px; color: rgb(143, 143, 143); font-family: Tahoma, "Microsoft Yahei", Simsun; background-color: rgb(255, 255, 255); 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